Enhancing Intracellular Uptake of Ivermectin through Liposomal Encapsulation
Meryem Kocas, Fumiyoshi Yamashita, Tansel Comoglu, Qiyue Zhang
AAPS PharmSciTech, doi:10.1208/s12249-025-03113-8
Ivermectin (IVM), an antiparasitic drug approved by the Food and Drug Administration (FDA), is widely used to treat several neglected tropical diseases, including onchocerciasis, helminthiases, and scabies. Additionally, IVM has shown potential as a potent inhibitor of certain RNA viruses, such as SARS-CoV-2. However, IVM is highly hydrophobic, essentially insoluble in water, which limits its bioavailability and therapeutic effectiveness. The use of liposomes as drug carriers offers several advantages, including enhanced solubility for lipophilic drugs, passive targeting of immune system cells, sustained release, and improved tissue penetration. To address the limitations of IVM, including its poor solubility and bioavailability, liposomal formulations were developed using a combination of soyphosphatidylcholine (SPC), dioleylphosphatidylcholine (DOPC), cholesterol (Ch), and diethylphosphate (DCP) in two distinct molar ratios (1.85:1:0.15 and 7:2:1) via the ethanol injection method. The physicochemical properties of the placebo and IVM-loaded liposomes were extensively characterized in our earlier study, including the particle size, polydispersity index, and zeta potential. The present work adds a deeper level of investigation into how to effect cellular uptake and cytotoxicity in vitro of both free IVM and IVM-loaded liposomes in Vero E6 cells. The half-maximal cytotoxic concentrations (CC 50 ) for free IVM and IVM-loaded liposomes were 10 μM and > 110 μM, respectively and the cellular uptake of IVM-loaded liposomes ranged from 13 to 60%, whereas free IVM showed a significantly lower uptake of only 2%. These results demonstrate that liposomal encapsulation effectively enhances IVM's cellular uptake while reducing its cytotoxicity, thus offering a promising strategy for improving the effectiveness of IVM.
Supplementary Information The online version contains supplementary material available at https:// doi . org/ 10. 1208/ s12249-025-03113-8. Author Contributions Meryem Kocas (ORCID: 0000-0002-4165-6191): The author contributed to the conception or design of the study; acquisition, analysis and interpretation of data for the study. She has also drafted or critically revised the manuscript for important intellectual content; and has agreed to be responsible for final approval of the version to be published and for all aspects of the publication. Fumiyoshi Yamashita (ORCID: 0000-0002-3503-8696): The author contributed to the conception or design of the study; acquisition, analysis and interpretation of data for the study. He has also drafted or critically revised the manuscript for important intellectual content; and has agreed to be responsible for final approval of the version to be published and for all aspects of the publication. Tansel Comoglu (ORCID: 0000-0002-4221-5814): The author contributed to the conception or design of the study; acquisition, analysis and interpretation of data for the study. She has also drafted or critically revised the manuscript for important intellectual content; and has agreed to be responsible for final approval of the version to be published and for all aspects of the publication. Qiyue Zhang (ORCID: 0000-0001-8440-2071): The author contributed to the conception or design of the study; acquisition, analysis and interpretation of data for the..
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"abstract": "<jats:title>Abstract</jats:title>\n <jats:p>Ivermectin (IVM), an antiparasitic drug approved by the Food and Drug Administration (FDA), is widely used to treat several neglected tropical diseases, including onchocerciasis, helminthiases, and scabies. Additionally, IVM has shown potential as a potent inhibitor of certain RNA viruses, such as SARS-CoV-2. However, IVM is highly hydrophobic, essentially insoluble in water, which limits its bioavailability and therapeutic effectiveness. The use of liposomes as drug carriers offers several advantages, including enhanced solubility for lipophilic drugs, passive targeting of immune system cells, sustained release, and improved tissue penetration. To address the limitations of IVM, including its poor solubility and bioavailability, liposomal formulations were developed using a combination of soyphosphatidylcholine (SPC), dioleylphosphatidylcholine (DOPC), cholesterol (Ch), and diethylphosphate (DCP) in two distinct molar ratios (1.85:1:0.15 and 7:2:1) via the ethanol injection method. The physicochemical properties of the placebo and IVM-loaded liposomes were extensively characterized in our earlier study, including the particle size, polydispersity index, and zeta potential. The present work adds a deeper level of investigation into how to effect cellular uptake and cytotoxicity <jats:italic>in vitro</jats:italic> of both free IVM and IVM-loaded liposomes in Vero E6 cells. The half-maximal cytotoxic concentrations (CC<jats:sub>50</jats:sub>) for free IVM and IVM-loaded liposomes were 10 μM and > 110 μM, respectively and the cellular uptake of IVM-loaded liposomes ranged from 13 to 60%, whereas free IVM showed a significantly lower uptake of only 2%. These results demonstrate that liposomal encapsulation effectively enhances IVM’s cellular uptake while reducing its cytotoxicity, thus offering a promising strategy for improving the effectiveness of IVM.</jats:p>\n <jats:p>\n <jats:bold>Graphical Abstract</jats:bold>\n </jats:p>",
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"volume": "26"
}